The GWAS data from this study connected the neuronal nAChR with cannabis-use disorder, however, it is unclear how they are connected.[1] It is possible that cannabis directly interacts with the nAChR, or that cannabis indirectly interacts with the nAChR.[1] A third possibility it that expression or regulation of the nAChR gene, CHRNA2, could be linked with the cannabinoid receptor 1 gene (CNR1), a possibility partially explored in the GWAS study.[1] It is partly this uncertainty that underscores the importance of validating GWAS data, such as the results of this study.[1]
[2] NCBI gene database. Gene ID: 1135. Available at: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=1135. Accessed November 2021.